the impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries

نویسندگان

sahar hatami

saeed zavareh

mojdeh salehnia

taghi lashkarbolouki

چکیده

background:   objective: the aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ala).   materials and methods: pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified–warmed pre-antral follicles were cultured individually with or without ala, followed by adding hcg to induce ovulation. the follicle growth, oocyte maturation, and embryo development were assessed. results: the diameter and development of follicles, oocyte maturation and embryo development rates were significantly higher in ala supplemented groups compared to the respective ala-free conditions groups. aforementioned parameters were significantly higher in vitrified-warmed follicles in comparison to follicles derived from vitrified-warmed ovaries. conclusion: these findings support a superior performance of pre-antral follicles when vitrified rather than when isolated from vitrified ovaries with regard to increasing the rates of developmental parameters. moreover, ala improves the in vitro maturation of pre-antral follicles in vitrified and non-vitrified samples. cryopreservation of ovarian tissues and pre-antral follicles is a promising prospect for preservation of women fertility.

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The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries

BACKGROUND Cryopreservation of ovarian tissues and pre-antral follicles is a promising prospect for preservation of women fertility. OBJECTIVE The aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ALA). MATERIALS AN...

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Total Oxidative status of Mouse Vitrified Pre-Antral Follicles with Pre-Treatment of Alpha Lipoic Acid

Background: Cryopreservation of pre-antral follicles is a hopeful technique to preserve female fertility. The aim of the present study was to evaluate reactive oxygen species (ROS) and total antioxidant capacity (TAC) levels of mouse vitrified pre-antral follicles in the presence of alpha lipoic acid (ALA). Methods: Isolated pre-antral follicles (140–150 µm in diameter) were divided into vitrif...

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عنوان ژورنال:
international journal of reproductive biomedicine

جلد ۱۲، شماره ۱، صفحات ۵۷-۰

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